2-FDCK HCl - An Overview
The lab claims fixation will result in the dyes to leak in the cells. I do think the belief is always that fixation could injury mobile membranes. I did locate a paper which describes fixation utilizing methanol or acetic acid, following staining which has a DCFDA by-product. DCFDA is the reagent that stains ROS from the kit ab113851, and I believe